Types of blood collection tubes and how to chooseIssuing time:2024-05-22 15:41 Choosing blood collection tubes mainly depends on whether you want to collect serum or plasma. Serum is the product of blood that has been allowed to clot. Plasma is the product of blood that has been prevented from clotting with an anticoagulant,while serum tubes contain silica nanoparticles or thrombin to induce clotting. When using plasma tubes, check that the anticoagulant is compatible with your down stream experiments.
In this short article, we explain different types of blood collection tube, including plasmatubes, serum tubes, and their key differences.
Blood Collection Tube Types
Serum vs. Plasma TubesThe first thing to figure out is whether you want to collectserum or plasma. That depends on whether or not you need to stop the blood from clotting. 1. Serum TubesThere are two main types of serum collection tubes that differ in how they activate blood clotting, using different clot activators. Silica-Based TubesThese tubes have silica nanoparticles, which activate clotting by activating coagulation factor XII. Some also have a gel to separate the serum.Those without the separating gel are usually more useful in sensitive diagnostic testing. Color of the cap: Red Clotting time: Approximately30 minutes. Thrombin-Based TubesThese tubes use thrombin to activate blood clotting. They’remainly used clinically for tests that are needed especially quickly. However, some serum components are a little less stable in these tubes. Color of the cap: Orange. Clotting time: Approximately 5 minutes. 2. Anticoagulant TubesThis is the category to consider if you need cells or plasma (acell-free liquid that still contains coagulation factors). EDTA Tubes (Purple)Contains EDTA anticoagulant, in the routine blood test, EDTA anticoagulant compared with other anticoagulants, the coagulation of bloodcells and the morphology of blood cells is less affected, so usually use EDTA salt (2K, 3K, 2Na) as anticoagulant. Note that these tubes containeither K2EDTA (dipotassium) or K3EDTA (tripotassium). Beware that K3EDTA ismore hyperosmolar due to the extra potassium ion—meaning it will concentrate blood samples more than K2EDTA via the hyperosmolar effect. Sodium Citrate Tubes (Light Blue)For coagulation and platelet function tests. Like EDTA, citrateremoves calcium from the blood by chelating it. Unlike EDTA, it’s reversible—socalcium can be added back to study coagulation under controlled conditions.Citrated plasma is also used to measure coagulation-relevant factors. Also, note that different citrate concentrations are availablefrom different companies, so check this before you buy them. Heparin Tubes (Green)Heparin is added to the blood collection tube. Heparin has the effect of antithrombin directly, which can prolong the clotting time of the specimen, and excessive heparin can cause the aggregation of leukocytes and cannot be used for leukocyte counting. Types of Blood Collection Tubes SummarizedFor more specific purposes, there are additional types of blood collection tubes out there, but hopefully, this has given you a handle on where to start. Remember to consider whether you want plasma or serum, and pick your tube accordingly. Note: Please remember to always wear protective equipment when working with blood, including safety goggles, gloves, and laboratory coats, andwork carefully to avoid contact with blood and blood exposure. It’s important to consider the safety of yourself and others in the lab.
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